Transient Expression Transient expression of triterpene biosynthetic enzymes from other plant species in N. benthamiana leaves can divert endogenous supplies of 2,3-oxidosqualene towards the production of new high-value triterpene products (Figure 1). This study explored the potential of plant-based system to produce an anti-human PD-1 monoclonal antibody (mAb), Pembrolizumab, in Nicotiana benthamiana. In many cases, researchers have relied on a single expression host for this purpose, namely Nicotiana benthamiana, because of its amenability to transformation and innate ability to support high levels of heterologous gene expression [ 4 ]. Nicotiana benthamiana as a transient expression host to produce … Efficient CELI endonuclease production in Nicotiana benthamiana … eCollection 2016. … [Abstract] Transient expression in tobacco plant (Nicotiana benthamiana) is used to determine. the subcellular location of a protein of interest when tagged with a reporter such as green. fluorescent protein (GFP), or to mass produce proteins without making transgenic plants. Transient expression A freshly prepared 50 mL aliquot of R. solanacearum suspension at OD 600 = 1 was used to drench the roots of N. benthamiana seedlings at 3 days after leaf infiltration with whitefly cDNA clones 2G4, 2G5, and 6A10 and empty vector (EV). Nicotiana benthamiana plant tissue culture in vitro, with transient expression of recombinant GFP, was obtained through shoot induction from leaf explants infected by a PVX-based vector. 2016 Jun 16;7:743. doi: 10.3389/fpls.2016.00743. ... Curtis WR: Agrobacterium-mediated viral vector-amplified transient gene expression in Nicotiana glutinosa plant tissue culture. Here, we review … Recent advances in pathway elucidation and host expression systems now allow metabolic engineering of plant metabolic pathways to produce “new-to-nature” derivatives with novel biological activities, thereby amplifying the range of industrial uses for … … transient expression

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